Glycoscience@Synchrotron: Synchrotron radiation applied to structural glycoscience

• Serge Pérez and
• Daniele de Sanctis

Beilstein J. Org. Chem. 2017, 13, 1145–1167, doi:10.3762/bjoc.13.114

• hydrolases; glycosyl transferases; kinetic crystallography; lectins; polysaccharides; powder diffraction; small-angle X-ray scattering; starch; synchrotron radiation; transporters; X-ray crystallography; Introduction Over the last decade, glycoscience has greatly benefited from the development of structural

• , explains the paucity of crystal structures deposited in the database [62][63][64][65][66][67][68][69][70][71][72]. This is even worse for those proteins involved in the transport of sugars (Figure 13) Kinetic crystallography Since the biological activity of many proteins is preserved in the crystalline

• state, the possibility to investigate the dynamic process of their mechanisms is absolutely intriguing. In kinetic crystallography, a biological reaction is initiated in the crystal and the fates of the transient species formed are followed by determining the structural changes. Depending upon the time